双链聚肌胞

生物活性靶点体外研究体内研究双链聚肌胞试剂级价格

双链聚肌胞

CAS号:42424-50-0
英文名:Polyinosinic-polycytidylic acid sodium salt
中文名:双链聚肌胞
CBNumber:CB2187556
分子式:C19H27N7O16P2
分子量:671.41
MOL File:42424-50-0.mol

双链聚肌胞化学性质

储存条件 :−20°C
溶解度 :H2O: soluble10mg/mL
形态 :lyophilized powder
水溶解性 :Water : 10 mg/mL (Need ultrasonic and warming)

安全信息

安全说明 :22-24/25
WGK Germany :3
RTECS号 :TR0175000

双链聚肌胞性质、用途与生产工艺

生物活性 Polyinosinic-polycytidylic acid sodium (Poly(I:C) sodium) 是双链 RNA 的合成类似物，是一种 TLR3 和视黄酸诱导型基因 I 受体 (RIG-I 和 MDA5) 的激动剂。Polyinosinic-polycytidylic acid sodium 可以用作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发生凋亡。
靶点

TLR3, RIG-I, MDA5, apoptosis

体外研究

Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours.
Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells.
Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1.
Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells.
Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use.

Cell Viability Assay

Cell Line:	WM793, WM278, WM239A, WM9 and 1205Lu cells
Concentration:	20 ng/mL
Incubation Time:	24 hours
Result:	Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.

Apoptosis Analysis

Cell Line:	1205Lu cells
Concentration:	200 ng/mL
Incubation Time:	24 hours
Result:	Induced apoptosis in 1205Lu cells.

RT-PCR

Cell Line:	1205Lu cells
Concentration:	3 ng/mL
Incubation Time:	24 hours
Result:	Induced IFN-β expression in melanoma cells.

Western Blot Analysis

Cell Line:	1205Lu cells
Concentration:	5 ng/mL
Incubation Time:	24 hours
Result:	Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.

体内研究

Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid.
用途一种可作为在TLR3水平对细胞信号转导进行研究的模型RNA的双链均聚物。也是一种TRIF依赖性toll样受体-3(TLR3)配体。