钙黄绿素乙酰甲酯
- CAS号:148504-34-1
- 英文名:CALCEIN-AM
- 中文名:钙黄绿素乙酰甲酯
- CBNumber:CB6304173
- 分子式:C46H46N2O23
- 分子量:994.86
- MOL File:148504-34-1.mol
- 熔点 :>250℃
- 沸点 :982.7±65.0 °C(Predicted)
- 密度 :1.49±0.1 g/cm3(Predicted)
- 折射率 :n
20/D 1.479 - 闪点 :85 °C
- 储存条件 :-20°C
- 溶解度 :DMSO: soluble10mg/mL, clear, colorless to slightly yellow
- 酸度系数(pKa) :2.66±0.50(Predicted)
- 形态 :powder
- 颜色 :Colorless
- 最大波长(λmax) :< 300 nm
- 生物领域应用 :Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes;multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease
钙黄绿素乙酰甲酯性质、用途与生产工艺
- 生物活性 Calcein-AM是用于测定细胞活力的可以渗透细胞的荧光染料。
-
体外研究
The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this microtest. Quantitation of killing and kinetic analysis is readily performed with the test system. Calcein-AM is pH independent, better retained and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane. From dying or damaged cells with compromised membrane integrity or from cells expressing multidrug resistance protein (MRP), unhydrolyzed substrates and their fluorescent products are rapidly extruded from cells. The calcein-AM assay has been used to assess the cell viability, cytotoxicity and tp quantitate apoptosis.
-
体内研究
Calcein-AM is found to be suitable for in vivo studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability.
- 更新日期:2024/04/30
- 产品编号:HY-D0041
- 产品名称:钙黄绿素乙酰甲酯 Calcein-AM
- CAS编号:148504-34-1
- 包装:500 μg(2 mg/mL * 250 μL in DMSO)
- 价格:2500元
- 更新日期:2024/01/25
- 产品编号:HY-D0041
- 产品名称:钙黄绿素乙酰甲酯 Calcein-AM
- CAS编号:148504-34-1
- 包装:100 μg(2 mg/mL * 50 μL in DMSO)
- 价格:1400元
- 公司名称:北京百灵威科技有限公司
- 联系电话:010-82848833 400-666-7788
- 电子邮件:jkinfo@jkchemical.com
- 国家:中国
- 产品数:96815
- 优势度:76
- 公司名称:北京偶合科技有限公司
- 联系电话:010-82967028 13552068683
- 电子邮件:2355560935@qq.com
- 国家:中国
- 产品数:12458
- 优势度:60
- 公司名称:凯试(上海)科技有限公司
- 联系电话:021-50135380
- 电子邮件:shchemsky@163.com
- 国家:中国
- 产品数:284
- 优势度:58
- 公司名称:凯试(上海)科技有限公司
- 联系电话:021-50135380
- 电子邮件:shchemsky@sina.com
- 国家:中国
- 产品数:15421
- 优势度:60
- 公司名称:大连美仑生物技术有限公司
- 联系电话:0411-62910999 13889544652
- 电子邮件:sales@meilune.com
- 国家:中国
- 产品数:4659
- 优势度:58
- 公司名称:南京奥生化学技术有限公司
- 联系电话:+86 (25) 82263158
- 电子邮件:
- 国家:中国
- 产品数:262
- 优势度:60
- 公司名称:上海源叶生物科技有限公司
- 联系电话:021-61312847 13636370518
- 电子邮件:shyysw007@163.com
- 国家:中国
- 产品数:4941
- 优势度:60
- 公司名称:上海阿拉丁生化科技股份有限公司
- 联系电话:18521735133 18521732826;
- 电子邮件:market@aladdin-e.com
- 国家:中国
- 产品数:25014
- 优势度:65
- 公司名称:上海喀露蓝科技有限公司
- 联系电话: 18149758185
- 电子邮件:sales-cpd@caerulumpharma.com
- 国家:中国
- 产品数:3423
- 优势度:58
- 公司名称:上海麦克林生化科技股份有限公司
- 联系电话:15221275939 15221275939
- 电子邮件:shenlinxing@macklin.cn
- 国家:中国
- 产品数:15878
- 优势度:55