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Fluorexon

Product Name:
Fluorexon
CAS No.
1461-15-0
Chemical Name:
Fluorexon
CBNumber:
CB0146553
Molecular Formula:
C30H26N2O13
Formula Weight:
622.54
MOL File:
1461-15-0.mol
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Fluorexon Property

Melting point:
200 °C
Boiling point:
662.7°C (rough estimate)
Density 
1.4388 (rough estimate)
refractive index 
1.5800 (estimate)
storage temp. 
Store at +5°C to +30°C.
solubility 
H2O: soluble
pka
6.67(at 25℃)
form 
Powder
color 
Yellow-orange to orange-brown
PH Range
Weak green ' uorescence (6.0) to strong green ' uorescence (7.2)
Water Solubility 
insoluble
λmax
494nm
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Calcium indicator; fluoride indicator; iron indicator; mercury indicator; detecting nucleic acids,proteins; treating osteoporosis; drug delivery systems
Major Application
Chemical-mechanical polishing, stabilizing liposomes, cell motility assay, detecting nucleic acids, virus, screening safety of gastric mucosa, MDR inhibitors, delivery, imaging atherosclerotic plaque
CAS DataBase Reference
1461-15-0(CAS DataBase Reference)
EPA Substance Registry System
Glycine, N,N'-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl)bis(methylene)]bis[N-(carboxymethyl)- (1461-15-0)
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Safety

Hazard Codes 
Xn
Risk Statements 
20/21/22-36/37/38
Safety Statements 
22-24/25-36/37/39-26
WGK Germany 
3
10
HS Code 
32041900
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N-Bromosuccinimide Price More Price(5)

Sigma-Aldrich Gold
Product number:
1.02315
Product name :
Calcein indicator
Purity:
for metal determination
Packaging:
1023150005
Price:
$171
Updated:
2019/12/02
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Buy
Cayman Chemical Gold
Product number:
16221
Product name :
Calcein
Packaging:
1g
Price:
$25
Updated:
2018/11/13
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Cayman Chemical Gold
Product number:
16221
Product name :
Calcein
Packaging:
5g
Price:
$44
Updated:
2018/11/13
Buy:
Buy
Cayman Chemical Gold
Product number:
16221
Product name :
Calcein
Packaging:
10g
Price:
$75
Updated:
2018/11/13
Buy:
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Cayman Chemical Gold
Product number:
16221
Product name :
Calcein
Packaging:
25g
Price:
$138
Updated:
2019/12/02
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Buy
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Fluorexon Chemical Properties,Usage,Production

Chemical Properties

orange crystals

Uses

Calcein is used for determination of Ca in biological systems. Indicator for the fluorimetric determination of calcium in presence of Mg by complexometric titration.

Uses

Calcein is a anthene that is commonly used for the fluorometric determination of calcium in solution. As this form of calcein is not membrane permeable, it can be used in assays that evaluate membrane integrity. Calcein can also be used as a fluorescent indicator for fluoride, iron, and mercury. [Cayman Chemical]

Purification Methods

Dissolve it in distilled H2O and acidify with dilute HCl to pH 3.5. Filter off the solid acid and wash it well with H2O. Redissolve ca 10g in 300mL H2O containing 12g of NaOAc. Precipitate it again by adding HCl, filtering and washing with H2O. Add the solid to 200mL of EtOH stir for 1hour and filter. Repeat the EtOH wash and dry the bright yellow solid in a vacuum. This acid decomposes on heating at ca 180o. See below for the preparation of the Na salt. [Diehl & Ellingboe Anal Chem 28 882 1956]. Altenatively dissolve it in H2O and acidify with 3N HCl to pH 3.5. Collect the solid and wash it with H2O. The air-dried precipitate is extracted with 70% aqueous EtOH, filtered hot and cooled slowly. Fine yellow needles of the acid crystallise out; they are filtered off and dissolved in the minimum quantity of 0.01N NaOH and re-precipitated by adding N HCl to pH 3.5. It is then recrystallised from 70% aqueous EtOH (3x). The final product (acid) is dried at 80o in a vacuum for 24hours, m >300o(dec). It contains one molecule of water per molecule of acid (C30H36N4O13.H2O). The product is pure as revealed by electrophoresis at pH 5.6 and 8.6, and by TLC in i-BuOH/i-PrOH/AcOH/H2O (60:60:5:5 by volume) or i-PrOH or pH 8.0 borate buffer. [Wallach et al. Anal Chem 31 456 1959.] The Na salt is prepared by dissolving the pure acid in H2O containing 2 mols of NaOH per mol of acid reagent and lyophilising. It complexes with Ca and Mg ions. [Beilstein 19 III/IV 4338.]

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Fluorexon Suppliers

Global(212)Suppliers
J & K SCIENTIFIC LTD.
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1461-15-0, FluorexonRelated Search:

Citric acid Ascoric Acid Folic acid Calcitonin salmon CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY BIS[N,N-BIS(CARBOXYMETHYL)AMINOMETHYL]FLUORESCEIN TETRASODIUM SALT FLUORESCEIN-BIS-METHYLIMINODIACETIC ACID (SODIUM) Fluorexon Hyaluronic acid Ethyl 2-(Chlorosulfonyl)acetate CARBOXYMETHYLCELLULOSE CALCIUM (1.5 G) (AS) FLUORESCEIN DIACETATE Calcitonin AZOMETHINE H Fluorescein LIGNOSULFONIC ACID, CALCIUM SALT Fluorescein Sodium Benzophenone imine
  • 2,2',2'',2'''-(((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(Methylene))bis(azanetriyl))tetraacetic acid
  • Calcein indicator for metal determination
  • Calcein, Suitable for fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
  • Fluorescein, 2′,7′-bis[[bis(carboxymethyl)amino]methyl]-
  • Spiro[isobenzofuran-1(3h),9′-[9h]xanthene], glycine deriv.
  • 2,7-bis[n,n-bis(carboxymethyl)aminomethylene]- Uo-rescein
  • Acetic acid, [(3′,6′-dihydroxy-2′,7′- Uorandiyl)bis-(methylenenitrilo)]tetra-
  • Nsc 298193
  • Spiro[isobenzofuran-1(3h),9′-[9h]xanthene], glycine derivative
  • Fluorescein, 2 0 ,7 0 -bis[[bis(carboxymethyl) amino]methyl]-
  • Spiro[isobenzofuran-1(3 h ),9 0 -[9 h ]xanthene], Glycine derivative
  • Acetic Acid, [(3 0 ,6 0 -dihydroxy-2 0 ,7 0 -fluor- andiyl)bis(methylenenitrilo)]tetra-
  • BIS[N,N-BIS(CARBOXYMETHYL)AMINOMETHYL]FLUORESCEIN
  • CALCEIN
  • CALCEINE
  • CALCEIN, INDICATOR
  • FLUORESCEIN-2,7-BIS-METHYLIMINODIACETIC SODIUM SALT
  • FLUORESCEIN COMPLEXON
  • FLUORESCEIN COMPLEXONE
  • FLUORESCEIN-METHYLENE-IMINODIACETIC ACID
  • FLUOREXON
  • FLUOREXONE
  • FLUREXONE
  • LABOTEST-BB LT00159821
  • 2,7-BLS[BIS(CARBOXYMETHYL)AMINOMETHYL]-FLUORESCEIN
  • 3,3'-BIS[N,N-BIS(CARBOXYMETHYL)AMINOMETHYL]FLUORESCEIN
  • 3,6-DIHYDROXY-2,4-BIS-N,N'-DI-(CARBOXYMETHYL)-AMINOMETHYL FURAN
  • FLUOREXON, INDICATOR GRADE
  • CALCEIN INDICATOR FOR THE COMPLEXOMETRY
  • CalceineIndicatorGr
  • CalceineIndicatorGr(FluoresceinComplexone)
  • CalceineIndicatorGr-(FluoresceinComplexon)
  • FLUORESCEIN COMPLEXONE GR
  • Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein~Fluorexon
  • Oftasceine
  • Glycine, N,N-(3,6-dihydroxy-3-oxospiroisobenzofuran-1(3H),9-9Hxanthene-2,7-diyl)bis(methylene)bisN-(carboxymethyl)-
  • CALCEIN(RG)
  • CALCEIN HYDRATE: FOR FLUOROMETRIC DETERMINATION OF CA
  • Fluorescein di-(methylimino diacetic acid)
  • Calcein(Fluorexone)
  • Calcein [for Fluorometric Determination of Ca]
  • Fluorescein-bis(methyliminodiacetic acid), Fluorexon, Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein
  • Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein, Calcein, Fluorescein Complexon
  • N,N'-[[3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-(carboxymethyl)glycine]
  • Calcein,Bis[N,Nbis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon
  • Calcein, indicator for metal titration
  • Bis-[di(carboxyMethylaMinoMethyl)]flurescein sodiuM salt
  • Fluorescein-2,7-bis-MethylaMinodiacetic acid sodiuM salt
  • CALCEIN INDICATOR FOR METAL DETERMINATIO
  • 7’-diyl)bis(methylene)]bis[n-(carboxymethyl)--2
  • glycine,n,n’-[(3’,6’-dihydroxy-3-oxospiro[isobenzofuran-1(3h),9’-[9h]xanthene]
  • N,N’-[(3’,6’-dihydroxy-3-oxospiro(isobenzofuran-1(3H),9’-(9H)xanthene)-2’,7’-diyl)bis(methyGlycine
  • Fluorescein-bis(methyliminodiacetic acid)
  • oftasccine
  • CALCEINCALCEINCALCEIN
  • Calcein, AR
  • 1461-15-0
  • C30H26N2O13