Chemical Properties
WHITE FLUFFY POWDER
Uses
2-Naphthyl acetate has been used as a substrate:
- in substrate sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to examine the composition of lipases in larvae and adult specimens
- in the preparation of zymograms to identify proteins with lipase activity
- to measure non-specific esterase activity and β-esterase enzyme activity
Uses
2-Naphthyl Acetate is used in the staining of gels for esterase activity in the biological laboratories. An esterase substrate.
Synthesis Reference(s)
Journal of the American Chemical Society, 91, p. 2162, 1969
DOI: 10.1021/ja01036a081
Purification Methods
Distil the acetate in a high vaccum and/or crystallise it from pet ether (b 60-80o) or dilute aqueous EtOH. The picrate has m 80o (from EtOH). [Beilstein 6 H 644, 6 I 313, 6 II 600, 5 III 2982, 6 IV 4267.]
