ML367 is a potent micromolar inhibitor of the ATPase family AAA domain-containing protein 5 (ATAD5). It blocks DNA repair pathways and general DNA damage responses, including UV-stimulated phosphorylation of RPA32 and phosphorylation of CHK1.
in vitro
ML367 (0-40 μM, 16 hours) inhibits FLAG-ATAD5 stabilization in HEK293T cells in the presence or absence of 20 μM 5-FUrd.
Western Blot Analysis
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Cell Line:
HEK293T cells
Concentration:
0-40 μM in the presence or absence of 20 μM 5-Furd
Incubation Time:
16 hours
Result:
Inhibited the increased ATAD5 protein levels induced by 5-Furd.
