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Nisin

Basic information Description References Safety Related Supplier

Nisin Basic information

Product Name:
Nisin
CAS:
1414-45-5
MF:
C143H230N42O37S7
MW:
3354.0705
EINECS:
215-807-5
Mol File:
1414-45-5.mol
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Nisin Chemical Properties

Boiling point:
2967℃
Density 
1.02 g/mL
Flash point:
>110°(230°F)
storage temp. 
2-8°C
form 
solution
Water Solubility 
Soluble in water
Merck 
13,6592
CAS DataBase Reference
1414-45-5
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Safety Information

Hazard Codes 
Xn
Risk Statements 
22-36/37/38
Safety Statements 
22-24/25-36-26
WGK Germany 
3
RTECS 
QU3340000
10

MSDS

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Nisin Usage And Synthesis

Description

As a ribosomally synthesized peptide, Nisin has broad-spectrum antibacterial activity that is produced by the Lactococcus lactis. In the food industry, Nisin is obtained from the cultivating of Lactococcus lactis on natural substrates, such as milk or dextrose. It serves as a natural, antibacterial, and toxicologically safe food preservative which is protect against many bacteria that are food-spoilage pathogens. As a food additive, Nisin is popularly used in food production, such as milk, meats, fast food, beverages, health care products and medicines, etc. It is effective to decrease sterilization time, lower temperature during food sterilization, improve food quality, lessen damage to nutrition, and extend shelf life by suppressing Gram-positive spoilage and pathogenic bacteria. Besides, it has been studied as a possible treatment for infections of Clostridium difficile combined with miconazole. It was originally isolated in the late 1930s and approved as an additive for food use in the USA in the late 1960s.
Nisin functions by binding to cell wall precursor lipid components of bacteria and disrupts cell wall production. Once introduced Nisin initially blends with Lipid II, a precursor molecule used to form bacterial cell wall, to create a complex. The complex penetrates the cell wall and breaks into the cytoplasmic membrane and form pores, through which the internal cytoplasmic content oozes out leading to bacterial inhibition or death.

References

https://en.wikipedia.org/wiki/Nisin
http://www.innovateus.net/food/natural-preservative-nisin
http://www.sigmaaldrich.com/catalog/product/sigma/n5764?lang=en®ion=US&
gclid=CPu1xf6aq9MCFcSOfgodvmcHZA

Chemical Properties

Crystals from ethanol. Derived from Streptococcus lactis Lancefield Group N.

Uses

Nisin is an antimicrobial agent derived from pure culture fermenta- tions of certain strains of streptococcus lactis lancefield group n. nisin preparation contains nisin, a group of related peptides with antibiotic activity. it is used to inhibit the outgrowth of clostridium botulinum spores and toxin formation in pasteurized cheese spreads and pasteurized process cheese spreads; pasteurized cheese spread with fruits, vegetables, or meats; and pasteurized process cheese spread with fruits, vegetables, or meats.

Uses

Nisin is a particularly effective bacteriosin against C. botulinum, is allowed in processed cheese food.

Uses

An antibiotic with bactericidal action

Definition

ChEBI: A type-A lantibiotic containing 34 amino acid residues (including lanthionine (Lan), methyllanthionine (MeLan), didehydroalanine (Dha) and didehydroaminobutyric acid (Dhb)) and five thioether bridges. It is obtained by fermentation of the bacterium L ctococcus lactis and shows particular activity against Clostridium botulinum. It is used in the production of various processed foods to suppress Gram-positive spoilage and pathogenic bacteria and so extend shelf life.

Purification Methods

This polypeptide from S. lactis is purified by crystallisation from 80% (v/v) EtOH and by countercurrent distribution. The synthetic polypeptide antibiotic can also be purified by preparative HPLC and assayed by HPLC on a Nucleosil 3007C18 (6 x 250mm) column using a MeCN—0.01M HCl gradient (30-50%), at 2%/minute, and flow rate of 1.5mL/minute to give a retention time of 8.1 minutes; or MeCN—0.3M guanidine-HCl gradient (30-50%), at 2%/minute, and flow rate of 1.5ml/minute to give a retention time of 10.9minutes. FAB-MS gave the pseudomolecular ion m/z at 3352.7 (M + H)+. It is soluble in dilute acid and is stable even on boiling. [Berridge et al. Biochem J 52 529 1952, synthesis by Fukase et al. Tetrahedron Lett 29 795 1988.]

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NisinSupplierMore

Shanghai JONLN Reagent Co., Ltd. Gold
Tel:
400-0066400;021-60496031
Email:
422131432@qq.com;422131432@qq.com
Products Intro:
Product Name:nisin from streptococcus lactis
CAS:1414-45-5
Purity:GR;AR;CP;HPLC Package:1g; 25g
Creative Peptides Gold
Tel:
Email:
info@creative-peptides.com
Products Intro:
Product Name:Nisin
CAS:1414-45-5
Purity:>98% Package:1g; 10g;100g;1kg Remarks:Peptide Inhibitors
XiaoGan ShenYuan ChemPharm co,ltd Gold
Tel:
0712-2580635- ;0712-2580635
Email:
1791901229@qq.com;1791901229@qq.com;
Products Intro:
CAS:1414-45-5
INTATRADE GmbH
Tel:
+49 3493/605464
Email:
sales@intatrade.de
Products Intro:
Product Name:Nisin
CAS:1414-45-5
Remarks:IS05774
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel:
821-50328103-801
Email:
3bsc@sina.com
Products Intro:
Product Name:Nisin
CAS:1414-45-5
Purity:99% HPLC Package:1Mg ; 5Mg;10Mg ;100Mg;250Mg ;500Mg ;1g;2.5g ;5g ;10g
Basic information Description References Safety Related Supplier