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Fluorescein isothiocyanate

Basic information Description Uses Safety Related Supplier

Fluorescein isothiocyanate Basic information

Product Name:
Fluorescein isothiocyanate
CAS:
27072-45-3
MF:
C21H11NO5S
MW:
389.38
EINECS:
248-207-7
Mol File:
27072-45-3.mol
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Fluorescein isothiocyanate Chemical Properties

Melting point:
>360 °C(lit.)
storage temp. 
2-8°C
form 
powder
Merck 
14,4089
BRN 
1407295
InChIKey
WBSWSUYBEIMVMI-UHFFFAOYSA-N
CAS DataBase Reference
27072-45-3(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5(or 6)-isothiocyanato- (27072-45-3)
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Safety Information

Hazard Codes 
Xn
Risk Statements 
42/43-36/37/38-20/21/22-42
Safety Statements 
22-36-26-24/25-45-36/37
WGK Germany 
3
10
HS Code 
29321900

MSDS

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Fluorescein isothiocyanate Usage And Synthesis

Description

Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)

  • Excitation source: 488 nm spectral line, argon-ion laser
  • Excitation wavelength: 494 nm
  • Emission wavelength: 518 nm
  • Extinction coefficient: > 70,000 M-1cm-1
  • Solubility: Soluble in aqueous buffers at pH > 6
  • Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0

Uses

  1. Label antibodies for use as immunofluorescent probes
  2. Label oligonucleotides for hybridization probes
  3. Detect proteins in gels and on Western blots

Chemical Properties

Dark yellow powder

Uses

For fluorescent labeling of proteins (e.g. immunofluorescence); protein labeling for gel chromatography.

Fluorescein isothiocyanate Preparation Products And Raw materials

Raw materials

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Fluorescein isothiocyanate SupplierMore

Tianjin heowns Biochemical Technology Co., Ltd. Gold
Tel:
400 638 7771
Email:
sales@heowns.com
J & K SCIENTIFIC LTD.
Tel:
400-666-7788 010-82848833-
Email:
jkinfo@jkchemical.com;market6@jkchemical.com
INTATRADE GmbH
Tel:
+49 3493/605464
Email:
sales@intatrade.de
TCI (Shanghai) Development Co., Ltd.
Tel:
021-67121386 / 800-988-0390
Email:
Sales-CN@TCIchemicals.com
Energy Chemical
Tel:
400-005-6266 021-58432009
Email:
sales8178@energy-chemical.com
Basic information Description Uses Safety Related Supplier