EC 4.2.1.1

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EC 4.2.1.1 Basic information

Product Name:
EC 4.2.1.1
CAS:
9001-03-0
MF:
NULL
MW:
0
EINECS:
232-576-6
Mol File:
Mol File
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EC 4.2.1.1 Chemical Properties

RTECS 
MT6446000
storage temp. 
2-8°C
solubility 
deionized water: soluble, clear to slightly hazy, colorless (Dissolve vial contents in 0.25mL of deionised water.)
form 
powder
color 
white
Water Solubility 
Soluble in water
Merck 
13,1822
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Safety Information

Hazard Codes 
B,Xi,T
Risk Statements 
36-41-40-37/38-24-20/22
Safety Statements 
26-45-36/37/39-53
WGK Germany 
3
10-21
HS Code 
35079090

MSDS

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EC 4.2.1.1 Usage And Synthesis

Purification Methods

Purify carbonic anhydrase by hydroxylapatite and DEAE-cellulose chromatography [Tiselius et al. Arch Biochem Biophys 65 132 1956, Biochim Biophys Acta 39 218 1960], and is then dialysed for crystallisation. A 0.5 to 1% solution of the enzyme in 0.05 M Tris-HCl pH 8.5 is dialysed against 1.75M solution of (NH4)2SO4 in the same buffer, and this solution is slowly increased in salt concentration by periodic removal of small amounts of dialysate and replacing with an equal volume of 3.5M (NH4)2SO4. The final salt concentration, in which the DEAE-cellulose fractions give beautiful birefringent suspensions of crystals, ranged from 2.4 to 2.7M and appeared first as fine crystals, then underwent transition to thin fragile plates. Carbonic anhydrase is a Zn enzyme which exists as several isoenzymes of varying degrees of activity [J Biol Chem 243 6474 1968, crystal structure: Nature, New Biology 235 131 1972; see also P.D. Boyer Ed. The Enzymes Academic Press NY, pp 587-665 1971].

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EC 4.2.1.1SupplierMore

J & K SCIENTIFIC LTD.
Tel:
010-82848833- ;010-82848833-
Email:
jkinfo@jkchemical.com;market6@jkchemical.com
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel:
821-50328103-801
Email:
3bsc@sina.com
Tianjin heowns Biochemical Technology Co., Ltd.
Tel:
400 638 7771
Email:
sales@heowns.com
Spectrum Chemical Manufacturing Corp.
Tel:
021-67601398-809
Email:
marketing_china@spectrumchemical.com;
ShangHai YuanYe Biotechnology Co., Ltd.
Tel:
021-61312847-
Email:
2797782341@qq.com
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