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Alkaline Phosphatase

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Alkaline Phosphatase Basic information

Product Name:
Alkaline Phosphatase
CAS:
9001-78-9
MF:
C21H36N8O6
MW:
496.56054
EINECS:
232-631-4
Mol File:
9001-78-9.mol
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Alkaline Phosphatase Chemical Properties

Density 
1.12 g/mL at 20 °C
storage temp. 
2-8°C
form 
suspension
Specific Gravity
1
color 
white
Water Solubility 
It is soluble in water.
Stability:
Stability Incompatible with strong oxidizing agents
EPA Substance Registry System
Alkaline phosphatase (9001-78-9)
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Safety Information

Hazard Codes 
B,Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-24/25-22-23
RIDADR 
UN 0029 1.1B
RIDADR 
UN 0030 1.1B
WGK Germany 
3
3-10
TSCA 
Yes
HazardClass 
1.1B
HazardClass 
1.1B
HS Code 
35079090

MSDS

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Alkaline Phosphatase Usage And Synthesis

Chemical Properties

solid

Uses

Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.

Uses

Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.

Purification Methods

The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]

Alkaline Phosphatase Preparation Products And Raw materials

Raw materials

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Alkaline PhosphataseSupplierMore

Hefei Bomei Biotechnology Co., Ltd. Gold
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13739298932
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531626407@qq.com
J & K SCIENTIFIC LTD.
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010-82848833- ;010-82848833-
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jkinfo@jkchemical.com;market6@jkchemical.com
Meryer (Shanghai) Chemical Technology Co., Ltd.
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21-61259100-
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sh@meryer.com
Energy Chemical
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021-58432009 / 400-005-6266
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Shanghai Longsheng chemical Co.,Ltd.
Tel:
021-58099637-8009;021-58099652-8005
Email:
sales@shlschem.com;bin.wu@shlschem.com
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