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网站主页 ANTI ACTIVE RAC1-GTP MOUSE MONOCLONAL ANTIBODY Anti Active Rac1-GTP Mouse Monoclonal Antibody
  • Anti Active Rac1-GTP Mouse Monoclonal Antibody

Anti Active Rac1-GTP Mouse Monoclonal Antibody

Anti Active Rac1-GTP Mouse Monoclonal Antibody
4800 1管 起订
湖北 更新日期:2022-04-18

武汉天德生物科技有限公司

非会员
联系人:乐***
手机:180***
邮箱:929***

产品详情:

中文名称:
Anti Active Rac1-GTP Mouse Monoclonal Antibody
英文名称:
Anti Active Rac1-GTP Mouse Monoclonal Antibody
保存条件:
-20℃
纯度规格:
1 mg/ml
产品类别:
单克隆抗体 活性单抗
Applications::
IP, IHC

Active Rac1 GTP [26903]                                                                                                                                              ¥:4800.00

[30 µL]

Catalog Number:  26903
Gene Symbol:  RAC1
Description:   Anti Active Rac1-GTP Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Immunogen:   Recombinant full length protein of active Rac1
Applications:   IP, IHC
Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                   1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgM
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:  
Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti Rac of the cell lysates used (5% of that used in the top panel).
Immunofluorescence:  
Immunofluorescence for the active Rac using Anti Active Rac1 GTP Mouse Monoclonal Antibody [26903] shows Rac GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. 
The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti mouse AlexaFluor488 and anti rabbit AlexaFluor594 at 1:1000.


Rac;Rac1;Rac1-GTP;Active Rac1-GTP;Rac1-GTP antibody;

公司简介

武汉戴安生物技术有限公司座落于武汉光谷生物城生物技术研究院,是一家由资深免疫学专家和海外留学人员创建的高科技生物技术企业。公司专注于体外诊断(IVD)试剂及其核心原料的研发生产及技术服务工作, 已成功建立了从IVD核心原料到试剂生产的研发生产路线,完成了原料开发-诊断试剂研发-诊断试剂放大生产的整个技术流程构建,拥有独立自主的抗原抗体的研发、放大生产技术平台及生化试剂大包装研发生产平台。   公司拥有完善的蛋白质表达体系,抗体制备体系,研发人员拥有十余年从业经验,技术积累深厚,设备精良完善,在重组蛋白质表达纯化、天然蛋白质提取、抗体研发和生产方面达到行业顶尖水平,也为客户提供专业的抗体定制及相关技术服务,包括科研用多克隆抗体和单克隆抗体定制,药物阻断用抗体筛选,中和抗体制备等技术服务。 我们秉承“精益求精,至善至美”的使命,追求“便捷,精确,真实”的客户体验,以创新推动产业发展,以实干满足市场需求。

成立日期 (6年)
注册资本 100万
员工人数 1-10人
年营业额 ¥ 300万-500万
经营模式 贸易,工厂,试剂,定制,服务
主营行业 生物化工,生化试剂,抗体,蛋白组学,诊断试剂

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