FITC标记的CD30抗体

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD30 / TNFRSF8 and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

品牌示例

Profile of anti-Human TNFRSF8 (CD30) reactivity on K562 cells analyzed by flow cytometry.

FITC标记的CD30抗体

背景

TNFRSF8/CD30 是一种 I 型跨膜糖蛋白，它是 TNFR 超家族成员。CD30 以前体蛋白形式合成，在将其作为 120-kda 的跨膜蛋白嵌入到质膜之前，需要经过广泛的翻译后修饰。CD30 的表达在活化 T 细胞中被上调，因此在相互作用过程中可触发协同刺激的信号转导通路。虽然它的表达通常局限于活化 T 细胞和 B 细胞亚群中，但 CD30表达在血液恶性肿瘤，如霍奇金淋巴瘤 (HL)、间变性大细胞淋巴瘤 (ALCL) 和成人 T 细胞白血病中高度上调，使其成为治疗干预的一个有吸引力的靶标。研究提示，在某些疾病背景下，CD30 通过募集 TRAF2 和 TRAF5 接头蛋白来驱动 NF-κB 活化、异常细胞生长和细胞因子生成。CD30 信号转导也受其胞外结构域的 TACE 依赖的蛋白溶解性裂解的调控，从而导致 CD30L 依赖的 CD30 + 细胞的活化减少。

简介

Mouse anti Human CD30, clone C03-8D3, recognizes the human CD30 cell surface antigen also known as TNFRSF8. CD30 is a 120 kDa, type I transmembrane glycoprotein of the tumour necrosis factor (TNF) receptor superfamily. CD30 was originally identified as a molecule expressed on Hodgkin and Reed Sternberg cells in Hodgkin’s disease but has subsequently been detected on certain non-Hodgkin's malignancies as well as some virally transformed cells. It is also expressed on a subset of activated human T-cells, and by a few extrafollicular T and B-cell blasts in normal lymph nodes.

下游应用

FITC标记的CD30抗体是一类可以特异性结合CD30的多克隆抗体，主要用于检测CD30的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。

检测原理

双抗体夹心法测定标本中CD30水平。用纯化的CD30抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CD30，再与HRP标记的CD30抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CD30呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中CD30浓度。