血卟啉二盐酸盐

应用生物活性体外研究体内研究血卟啉二盐酸盐试剂级价格

血卟啉二盐酸盐

CAS号:17696-69-4
英文名:HEMATOPORPHYRIN DIHYDROCHLORIDE
中文名:血卟啉二盐酸盐
CBNumber:CB9281835
分子式:C34H39ClN4O6
分子量:635.16
MOL File:17696-69-4.mol

血卟啉二盐酸盐化学性质

储存条件 :Inert atmosphere,Room Temperature
溶解度 :DMSO (Slightly), Methanol (Slightly)
形态 :Solid
颜色 :Very Dark Red
水溶解性 :Insoluble in water. Soluble in pyridine (5%).
Merck :14,4636
BRN :78957
稳定性 :Hygroscopic
EPA化学物质信息 :21H,23H-Porphine-2,18-dipropanoic acid, 7,12-bis(1-hydroxyethyl)-3,8,13,17-tetramethyl-, dihydrochloride (17696-69-4)

安全信息

危险类别码 :33
RTECS号 :TS5505000
TSCA :Yes
毒性 :mouse,LD50,intraperitoneal,169mg/kg (169mg/kg),BEHAVIORAL: TREMORBEHAVIORAL: CHANGES IN MOTOR ACTIVITY (SPECIFIC ASSAY),Bollettino Chimico Farmaceutico. Vol. 117, Pg. 145, 1978.

血卟啉二盐酸盐性质、用途与生产工艺

应用有研究表明血卟啉二盐酸盐可用于制备具有高结晶速率的树脂组合物，其包含聚呋喃二甲酸亚烷基酯(polyalkylenefurandicarboxylate)树脂和卟啉化合物。该聚呋喃二甲酸亚烷基酯树脂优选为聚呋喃二甲酸亚丁基酯树脂。该卟啉化合物优选为血卟啉二盐酸盐(hematoporphyrindihydrochloride)。
生物活性 Hematoporphyrin dihydrochloride (Hematoporphyrin IX dihydrochloride) 是一种光敏剂，是血红素结合蛋白亲和色谱底物。当暴露于红光下时，Hematoporphyrin dihydrochloride 可以诱导 U87 胶质瘤细胞凋亡，并降低体内肿瘤的生长。

体外研究

Hematoporphyrin (20-120 nM; 60 min) dose-dependently inhibits cell viability in U87 and U251 glioma cells, with IC ₅₀ s of 85 and 166 nM, respectively.
Hematoporphyrin (85 nM; 60 min) induces cell apoptosis via induction of ROS in U87 cells.
Hematoporphyrin (85 nM; 60 min) induces morphological changes of U87 cells under the red light, including shrinking, fragmentation.

Cell Viability Assay

Cell Line:	U87 and U251 cells
Concentration:	20, 40, 60, 80, 100, 120 nM
Incubation Time:	60 min
Result:	Inhibited cell viability in a dose-dependent manner. Was more effective under the red light than white light.

Apoptosis Analysis

Cell Line:	U87 cells
Concentration:	85 nM
Incubation Time:	60 min
Result:	Induced apoptotic nuclei in U87 cells with low cell density. Induced the ROS and decreased the mitochondrial membrane potential.

体内研究

Hematoporphyrin (5-10 mg/kg; i.p. for 2 months) with the irradiation of red light rapidly decreases the tumor size of rats, due to necrosis caused both by direct action of the photoactivated porphyrin on the tumor cells and by secondary effects on blood vessels.

Animal Model:	Wistar albino rats of both sexes (20 d; 60-80 g) bearing a subcutaneous solid Yoshida hepatoma AH-130
Dosage:	5, 10 mg/kg
Administration:	I.p. daily during the initial 10 days and biweekly for the next 2 months
Result:	No tumor could be palpated a few days after exposure of the rats to light. The skin healed completely and regrowth of the hair occurred. Massive coagulation necrosis of the tumor 24 h after phototreatment (×40).