Helicid is the active ingredient isolated from the fruit of proteaceae plant Helicia nilagirica, with its chemical structure being similar to gastrodin (hydroxymethyl benzene - O-β-D-glucopyranoside). Pharmacological and clinical studies have shown that helicid has similar effects on the central nervous system with gastrodin, but its sedative, euthanasia and analgesic effect is stronger than gastrodin. The efficacy on the treatment of neurosis efficacy is definite without rebound insomnia and drug dependence. Therefore, it has excellent clinical value. Its fruit tablets have been used in clinical.
HPLC method
(1) Chromatographic conditions
Column: YWCT-C18 (25cm × 2.6mm, 10μm) high pressure grouting method. Flowing phase: methanol-water (20:80); flow rate: 0.7 ml / min; column temperature: room temperature; UV detection wavelength: 270 nm; paper speed: 2.5 mm / min.
(2) Reference solution preparation
Precisely weigh control reference substance of helicid; prepare 1 mg/mL solution using 20% methanol. Precise weigh Bergenin; dissolve in 20% methanol; apply ultrasonic solubilization, dub bergenin into about 2mg / ml internal standard solution.
(3) Preparation of sample solution
Accurately weighed dried helicid powder of about 1g, and put it into 25ml volumetric flask; add 20 mL methanol for soak of 0.5 h; ultrasonic extraction 80min, cool, set volume into 25 mL with methanol; filter and filtrate will become the sample solution.
(4) Determination
Take helicid reference solution in 5ml volumetric flask, add bergenin internal standard solution of 1.5ml; diluted with 20% methanol to the mark, mix; take 5 mL sample solution, volatilize; add 1. 5mL the internal standard solution; dilute with 20% methanol to the mark, mix well. Take 5μl of both reference substance and sample solution, inject, determine according to the chromatographic conditions; calculate the content with peak area ratio.
(5) Measurement results
Use the above internal standard method to measure the content of helicid in Samples I and II, and the results were 0.963% and 0.928%.
Its chemical structure is similar to Gastrodia; have a strong analgesic, sedation and euthanasia effect. It is used for the treatment of neurasthenia, neurasthenic syndrome, functional disorders, vascular headache and trigeminal neuralgia. It has remarkable effect on neurasthenic headache, dizziness and sleep disorders.
Figure 1 the process of purification of helicid from the fruit of proteaceae plant Helicia nilagirica
